Volyn Regional Children's Territorial Medical Center

Laboratory of Medical Genetics, Immunology,
Endocrinology and Clinical Biochemistry

It is well-known that who diagnoses well also treats well. Having modern laboratory equipment is vitally important; it is a representation of the situation in medicine. Unlike usual clinical examinations carried out in laboratories to ensure a minimum of necessary testing scope, our Laboratory of Medical Genetics, Immunology, Endocrinology and Medical Biochemistry conducts different more specific tests.

We traditionally do hormonal examinations in case of thyroid diseases, infertility, menstrual cycle disorders, and miscarriages. Informative are oncomarkers – tests to determine specific substances produced by tumors. The laboratory can diagnose antibodies to TORCH-infections, tuberculosis, lamblias, helicobacters, hepatitis. Pregnant women are proposed examinations to reveal birth defects in their fetuses. In the laboratory it is possible to determine karyotype (cytogenetic test of quantity and quality of chromosomes).

In the laboratory a number of immunological tests can be performed which gives a possibility to estimate immunological status of a patient, his resistibility to external exposures and diseases, identify autoimmune or allergic processes, conduct immune marker analysis of leukemias.

High precision and quality immunological tests are performed using the contemporary flow cytometry FACS Calibur analyzer which is confirmed by the certificate given by Becton, Dickinson and Company (the analyzer producer). To guarantee the laboratory processes the lab is equipped by Leits and Zeiss binocular light microscopes, photometrical apparatuses Stat FAX-303 and Stat FAX-1904, multifunctional analyzer Victor. Below there is a list of tests which can be done in our laboratory.

Test done by immune-enzyme analysis method.

Thyroid hormones (T3, T4, thyreoglobulin antibodies, microsomal antigen antibodies, thyreoglobulin).
Hormones of hypophysis anterior lobe (prolactin, thyrotropic hormone, neonatal thyrotropic hormone, follicle-stimulating hormone, luteinizing hormone, adrenocorticotropic hormone).
Hormones of adrenal glands (cortisol, dehydroepiandrosterone sulfate).
Sex hormones (testosterone, 5-a-dihydrosterone, estradiol, progesterone, 17-a-OH-progesterone).
Antibodies to TORCH-infections: toxoplasmosis, cytomegalovirus, clamydia, herpes simplex virus, rubella.
Identification of cytomegalovirus protein.
Identification of avidity to rubella.
Antibodies to candida.
Antibodies to mycoplasma.
Antibodies to trichomonad.
Total antibodies to tuberculosis.
Antibodies to helicobacter pilori.
Antibodies to lamblia.
Antibodies to nuclear form of Epstein-Barr virus.
Antibodies to hepatitis A, B, C, D, E.
Identification of C-peptide level.
Antibodies to phospholipin component.
Carcinoembryonal antigen.
Prostatic specific antigen.
CA-125,CA-153, CA-199.
Identification of antisperm antibodies.
Identification of a-fetoprotein, PAPP, chorionic gonadotropin.

Genetic tests.

Screening tests (hereditary metabolic diseases).
Identification of chlorine ions in sweat.
Mass screening for PKU.
Screening for congenital thyreoid deficiency.
Testing of chromosomes in peripheral blood lymphocytes at metaphase stage.
Identification of X-chromatin in cells of mouth mucous membrane.
Identification of hemophilia type.

Immunological tests.

Humoral immunity (immunoglobulin A, M, G, E).
Circulating immune complexes.

Immunological tests with the help of the flow cytometry FACS Calibur analyzer (Becton Dickinson). Identification of CD diagnostic clusters: CD₃, CD₄, CD₅, CD₇, CD₈, CD₁₀, CD₁₃, CD₁₄, CD₁₉, CD₂₀, CD₂₂, CD₂₅, CD₂₈, CD₃₃, CD₄₅, CD₃₄, CD₁₆₊₅₆, CD₉₅.

Immune status in children and adults.
Monitoring of immunodeficiency state.
Diagnosis of autoimmune pathology (HLA-B27).
Immune status in transplantology.
Monitoring of treatment process.
Immunophenotyping in leukemias and lymphomas.
Identification of antitumoral factors.

In January 2008 the laboratory began making tests using the polymerase chain reaction technique (PCR). This technique has been used in the world since 1993; for its development Dr. Kary Mullis was awarded with the Nobel Prize because it was an extraordinary event in molecular biology. Now this method is available in the Volyn Regional Children’s Territorial Medical Center as well.